High Performance Liquid Chromatographic Method for Determination of Colistin Sulfate and its Application in Medicated Premixand Animal Feed
نویسنده
چکیده
The aim of the present study was to develop and validate an inexpensive and simple high performance liquid chromatographic (HPLC) method for the determination of colistin sulfate. Separation of colistin sulfate was achieved on a ZORBAX Eclipse XDB-C18 column using UV detection at λ=215 nm. The mobile phase was 30 mM sulfate buffer (pH 2.5): An excellent linearity (r=0.998) was found in the concentration range of 25 400 μg/mL. Intraday and inter method (%RSD, n=3) were less than 7.9%. validated method was applied to determination of the colistin sulfate in medicated premix and animal feed recovery of colistin from animal feed was satisfactorily ranged from 90.92 to 93.77%. The results demonstrated that the HPLC method developed in this work is appropriate for direct determination of colistin sulfate in commercial medicated premixes and animal feed. Keywords—Colistin sulfate, HPLC, medicated premix feed.
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